Intact protein analysis by QTOF high resolution mass spectrometry
As described above, the mass of intact proteins can be determined by QTOF high resolution mass spectrometry with high accuracy. This is a state-of-the art technique to confirm and further resolve the mass as observed by SDS-PAGE or HP-SEC. The deconvoluted mass spectrum of a glycosylated heavy chain is shown here as an example:
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Other examples are given in our publications on human transferrin and intact IgG. We also have experience with serum albumin and many confidential protein conjugates and fusion proteins from different clients in pharma industry.
Intact human transferrin analysis
References:
Jacobs JFM et al. Clin Chim Acta 2016; 461, 90–97.
Van Scherpenzeel M et al. Trans Res 2015; 166:639–649.
